Timothy G. Strein

Professor of Analytical Chemistry
Presidential Professor

Chair, Department of Chemistry
Bucknell Univeristy
Lewisburg, PA 17837

(570) 577-1641, strein@bucknell.edu

 
BS North Carolina State University, 1988
 
PhD Penn State University, 1992
Electrochemistry at Microvoltammetric Electrodes with Dr. Andrew G. Ewing
 
Camille and Henry Dreyfus Postdoctoral Fellow, Bucknell University 1992-1994
Capillary Electrophoresis of Physiological Fluids with Dr. Hans Veening
 

Research Interests: In-capillary chemical reations, electrophoretic stacking phenomena in CE, chiral separations with CE, bile salt micellar structure, aqueous NMR, microvoltammetric electrodes.

Areas of Teaching Expertise: Analytical Chemistry, Chemical Equilibria, Instrumental Analysis, Forensic Chemistry, General Chemistry.

Service Interests: Promotion of undergraduate research, Departmental Graduate (MS) Coordinator (1998-2006), member Bucknell Graduate Council (2002-), Program Co-Chair MARM'06, Chair, Department of Chemistry (2010-), Acting Chair (2006-2007).

Department of Chemistry, Bucknell University, Lewisburg, PA 17837

Bucknell Chemistry Department Web Site

Research Interests | External Funding | Recent Publications | Teaching Interests | Former Group Members

Research Interests: Bioanalytical Chemistry

The Strein group is interested in both the fundamentals and the applications of analytical techniques and technologies. A good deal of our work falls within broad area of separation science but we are also dabbling in electrochemistry. On the separations side, we work with the capillary electrophoresis (CE) and are interested in separation schemes for the analysis of clinical biofluids and commercial beverages, the fundeamentals of stackiong phenomena, and mechanisms for chiral separation by CE. We have also done some collaborative work on the separation of dedrimers and the determination of mobilities in biological tissues.

One current (2014) project focuses on gaining a fundamental understanding of, as well as exploiting the capabilities of, CE to perform in-line chemical reactions between small molecules or ions. We are investigating techniques for electrophoretic mixing and for both field-enhanced and transient isotachophoretic stacking of either the reactants or the products of in-line reactions. We have been using the Jaffe reaction between picrate and creatinine as a model system for most of these studies, but are also investigating reactions of anti-oxidants.

A second current project involves CE-based chiral separations enabled by bile slat micellar chiral additives to CE background buffers. We are particularly interested in understanding how micelles formed by bile salts such as sodium cholate, deoxycholate and chenodeoxycholate act as chiral selectors. This interest has spurred a collaborative project with the Rovnyak group in which NMR is being used to understand the dynamics of chiral bile salt micelle formation and the interaction of bile salt micelles with model chiral analytes.

An important part of our research efforts is the collaborative nature of student/faculty research at Bucknell. Typically, between 3 and 6 very talented and energetic students make up the research group. Current students in the group include:

Nick Doyle
2nd year MS Student

Understanding bile salt structure using NMR, MS and PCA
Shauna_2014

Shauna Anderson
1st year MS Student

ITC with cholate and deoxycholate systems, bile salt hydrogels

Maria_2014 Maria Jones
Senior Biochemistry Major
Understanding electrophoretic stacking dynamics with in-line Jaffe system
Ross_2014

Ross Pirnie
Sophomore Biochemistry Major

Chiral MEKC separtaions with bile salts, NMR taurodeoxycholate systems

Libby_2014

Libby Seidell
Sophomore Chemistry Major

Electrophoretic Stacking with Jaffe and other systems
Abby_2014

Abigail Kreznor
First year Chemistry Major

EMMA and hydroxide-mediated stacking with the Jaffe system

For a list of former group members, and where they are now, click here (continually under construction).

External Funding

NSF-RUI Grant #CHE-1153052 (with David Rovnyak) “Molecular Characterization of Aggregation and Guest-Host Solvation by Bile Acids” $159,814, 2012-2015.

NIH AREA Grant #1 R15 EB003854-02 "Bioanalytical CE: Mixing, Reacting, Separating, Stacking" 2007-2011, $218,899

ACS-PRF "Examining hydrodynamic and solubilization properties of micelles formed by chiral amphiphiles with NMR" with David Rovnyak, 2007-2011 $55,000

NIH AREA Grant #1 R15 EB003854-01 "Polarity Switching and Stacking in Bioanalytical CE" 2004-2007, $139,400.

NSF-MRI Grant # CHE-0521108 "Acquisition of a High-Resolution NMR Spectrometer to Advance Faculty and Undergraduate Research," with David Rovnyak (principal author) and others, 2005-2008, $475,000.

Henry Dreyfus Teacher/Scholar Award TH-98-025, "Activation, characterization and application of carbon fiber microelectrodes," 1998-2203, $60,000.

Research Corporation Grant #CC4333, renewal of "Capillary electrophoresis of physiological fluids: Small molecules to proteins," 1997-1999, $27,000.

NSF-ILI grant DUE-9551135, "Enhancement of the Undergraduate Chemistry Experience with Bench-Top GC-MS," 1995-1998 $24,732.

Council on Undergraduate Research Academic-Industrial Undergraduate Research Partnership (AIURP) Award, for summer research student Cozette Cuppett.
1996 $3,000.

Research Corporation Grant # CC3788, "Capillary electrophoresis of physiological fluids: Small molecules to proteins," 1995-1997 $28,500.

Camille and Henry Dreyfus Supplemental Grant number SL-94-02, "Bioanalytical Chemistry at Bucknell" 1994, $10,000.

Camille and Henry Dreyfus Start-up Grant number SU-94-011, "Development and use of capillary electrophoretic and microvoltammetric methods of analysis, and their application to biologically interesting samples," 1994, $12,500.

Society for Analytical Chemists of Pittsburgh Starter Grant, "Laser Activation at Carbon Fibers," 1994, $10,000.

NSF-ILI grant number DUE-9352607, "Capillary Electrophoresis in the Undergraduate Curriculum," T. G. Strein (principal author) with H. Veening, J. Swan, and C. Clapp, 1993-1996, $19,020.

 

Recent Publications

Guy, Y.; Faraji, A.H.; *Gavigan, C., Strein, T.G. and Weber, S.G. “Iontophoresis From a Micropipette into a Porous Medium Depends on the Zeta-Potential of the Medium”, Analytical Chemistry, 2012, 84, 2179-2187  (DOI: 10.1021/ac202434c).

Faraji, A.H.; Cui, J.J.; Guy, Y.; Li, L.; *Gavigan, C.A. Strein, T.G. and Weber,S.G. “Synthesis and Characterization of a Hydrogel with Controllable Electroosmosis: A Potential Brain Tissue Surrogate for Electrokinetic Transport” Langmuir, 2011, 27 (22), pp 13635-13642 (DOI: 10.1021/la202198k).

Alcala, M.A.; Shade, C.M.; Uh, H.; Kwan, S.Y.; Bishof, M.; Thampson, Z.P.; Golick, K.A.; *Meier, A.R.; Strein, T.G.; Bartlett, D.L.; Modzelewski, R.R.; Lee, Y.J.; Petoud, S.; Brown, C.K. “Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes” Biomaterials, 32, 2011, 9343-9352. (DOI:10.1016/j.biomaterials.2011.07.076)

Stahl, J. W.; Catherman, A.D.; Ranasinghe S.K.; Strein, T.G. "Investigating the Effects of Conductivity of Zone Overlap with EMMA:  Computer Simulation and Experiment" Electrophoresis 32, 2011, 1492-1499. (DOI: 10.1002/elps.201000451)

Merola, E.T.; Catherman, A.D., Yehl, J.B.; Strein, T.G. "Determination of Total Antioxidant Capacity of Commercial Beverage Samples by Capillary Electrophoresis using 2,6-Dichloroindophenol" J. Ag and Food Chem. 57(15), 2009, 6518-6523. (DOI: 10.1021/jf901214r)

Mason, P.; Strein T.G. “ In-capillary determination of creatinine with electrophoretically mediated microanalysis: characterization of the effects of reagent zone and buffer conditions ” Journal of Chromatography, A12162009,154-158. (DOI: 10.1016/j.chroma.2008.11.033)

Hebling, C.M.; Eckenroad, K.W.; Rovnyak, D.; and Strein, T.G. “Sodium Cholate Aggregation Observed by Nuclear magnetic Resonance Spectroscopy of the Probe Molecule (R,S) 1,1'-bunapthyl-2,2'- dihydrogenphophate (BNDHP)” Langmuir,  242008, 13866-13874. (DOI:10.1021/la802000x)

Eckenroad, K.W.; Thompson, L.E.; Strein, T.G.; Rovnyak, D. "Proton NMR assignments for R,S-1,1-binapthyl (BN) and R,S-1,1-binaphthyl-2,2-diyl hydrogen phosphate (BNDHP) interacting with bile salt micelles" Magnetic Resonance in Chemistry, 45, 2006, 72-75. (DOI: 10.1002/mrc.1916).

Sanders, B.D.; Slotcavage, R.L.; Scheerbaum, D.L.; Kochansky, Christopher J; .Strein, T.G. "Increasing the Efficiency of In-Column Reactions via Rapid Polarity Switching" Analytical Chemistry, 77, 2005, 2332-2337. (DOI: 10.1021/ac048595u)



Teaching Interests

Teaching Area: Anlaytical Chemistry

CHEM231 Analytical Chemistry I: Quantitative Analysis course for sophomore and junior Chemistry, Biology and Chemical Engineering majors
CHEM332 Analytical Chemistry II: Instrumental Analysis course for junior and senior level Chemistry and Biochemistry majors
CHEM337 Special Topics in Analytical Chemisty including forensic chemistry, DNA analysis, and chemistry on Mars.
CHEM403 Research in Chemistry Capstone Course for Seniors
CHEM 386/686 Seminar Course in Separations Science for MS students and upper level undergraduates
CHEM340 Lab Biophysical Chemistry lab for junior/senior BS biochemistry majors and BA chemsitry majors
CHEM201
General Chemistry: College Chemistry course for sophomore engineers
FOUN098,090 Forensic Chemistry Foundation Seminar

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Bucknell Chemistry Department Web Site

Cell Biology / Biochemistry program

last edited 06/14